Forensic analysis of Chrysomya megacephala growth patterns for precise post-mortem interval estimation
DOI:
https://doi.org/10.33687/ricosbiol.02.02.0024Keywords:
Forensic Entomology, Chrysomya megacephala, Post-Mortem Interval (PMI), Decomposition StagesAbstract
The study investigates growth dynamics at critical stages of decomposition by Chrysomya megacephala - Fresh, Bolting, Decay, Actively Decay, and Dry. Meticulous variation in weight under environmental conditions during each stage could reflect distinct growth patterns influenced by temperature, humidity, and availability of substrates to improve the forensic accuracy of PMI estimation. Early larval stages such as Fresh and Bolting recorded moderate weight gain, but the peak feeding activity occurred at the Decay and Actively Decay stages where the weights stabilized due to decreased tissue availability. The existence of high weights during favorable conditions, especially a fresh weight of 10.485 mm on D13, emphasizes how environmental factors promote the rapid growth of larvae, particularly in nutrient-rich environments. Findings show that larvae of Chrysomya megacephala are sensitive to environmental changes, thus making their growth patterns very useful in forensic practices. In fact, this study further underpins the species as a strong candidate for being a useful indicator of PMI; because the growth stages tracked against specific conditions allow forensic investigators to make more precise calculations of PMI, presenting a more refined tool in the criminal investigation process.
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References
Bansode, S. A., More, V. R., Zambare, S. P., and Fahd, M. (2016). Effect of constant
Temperature (20 0C, 25 0C, 30 0C, 35 0C, 40 0C) on the development of the Calliphorid fly of forensic importance, Chrysomya megacephala (Fabricus, 1794). Journal of Entomology and Zoology Studies, 4(3), 193-197.
Badenhorst, R., and Villet, M. H. (2018). The uses of Chrysomya megacephala (Fabricius,
1794) (Diptera: Calliphoridae) in forensic entomology. Forensic sciences research, 3(1), 2-15.
Chaiwong T, Srivoramas T, Sueabsamran P, Sukontason K, Sanford MR., and Sukontason KL. (2014). The blow fly, Chrysomya megacephala, and the house fly, Musca domestica, as mechanical vectors of pathogenic bacteria in Northeast Thailand. Tropical Biomedicine, 31(2), 336-346.
Guiadem, L. S., Dhuideu, C. T., Bozdoğan, H., Mballa, A. N., and Kekeunou, S. (2023).
Spatio-temporal distribution of Chrysomya megacephala (Diptera: Calliphoridae), mechanical vector of infectious diseases in the city of Yaoundé (Centre-Cameroon). International Journal of Tropical Insect Science, 43(2), 617-627.
Jeong, Y., Weidner, L. M., Pergande, S., Gemmellaro, D., Jennings, D. E., and Hans, K. R.
(2022). Biodiversity of Forensically Relevant Blowflies (Diptera: Calliphoridae) at the Anthropology Research Facility in Knoxville, Tennessee, USA. Insects, 13(2), 109.
Mendonça, P. M., Dos Santos‐Mallet, J. R., and De Carvalho Queiroz, M. M. (2012).
Ultrastructure of larvae and puparia of the Chrysomya megacephala Chrysomya megacephala (Diptera: Calliphoridae). Microscopy Research and Technique, 75(7), 935-939.
Ngoen-Klan, R., Moophayak, K., Klong-Klaew, T., Irvine, K. N., Sukontason, K. L.,
Prangkio, C., and Sukontason, K. (2011). Do climatic and physical factors affect populations of the blow fly Chrysomya megacephala and house fly Musca domestica? Parasitology Research, 109, 1279-1292.
Qu, Y., Wang, B., Deng, J., Feng, Y., Pi, Z., Ren, L., and Cai, J. (2023). Geographical
Distribution and Multimethod Species Identification of Forensically Important Necrophagous Flies on Hainan Island. Insects, 14(11), 898.
Sawaby, R. F., Hamouly, H. E., and Abo-El Ela, R. H. (2018). Diagnosis and keys of the main
Dipterous families and species collected from rabbit and guinea pig carcasses in Cairo, Egypt. The Journal of Basic and Applied Zoology, 79, 1-14.
Sebastião, M., and Prado e Castro, C. (2019). A preliminary study of carrion insects and their
Succession in Luanda, Angola. Journal of Medical Entomology, 56(2), 378-383.
Sharma, A., and Bala, M. (2016). Case study and PMI estimation of male corpses from
Ludhiana, Punjab, India: An implication of ADH method. Indian Journal Forensic Medicine and Toxicology, 10(2), 28-33.
Sontigun, N., Sukontason, K. L., Klong-Klaew, T., Sanit, S., Samerjai, C., Somboon, P., and
Sukontason, K. (2018). Bionomics of the oriental latrine fly Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae): temporal fluctuation and reproductive potential. Parasites & vectors, 11(1), 1-12.
Vilte, R., Gleiser, R. M., and Horenstein, M. B. (2020). Necrophagous fly assembly:
Evaluation of species bait preference in field experiments. Journal of Medical Entomology, 57(2), 437-442.
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